Development and application of catalytic tyrosine modification was written by Sato, Shinichi;Tsushima, Michihiko;Nakamura, Kosuke;Nakamura, Hiroyuki. And the article was included in Yakugaku Zasshi in 2018.Recommanded Product: 18393-54-9 This article mentions the following:
The chem. labeling of proteins with synthetic probes is a key technique used in chem. biol., protein-based therapy, and material science. Much of the chem. labeling of native proteins, however, depends on the labeling of lysine and cysteine residues. While those methods have significantly contributed to native protein labeling, alternative methods that can modify different amino acid residues are still required. Herein we report the development of a novel methodol. of tyrosine labeling, inspired by the luminol chemiluminescence reaction. Tyrosine residues are often exposed on a protein surface and are thus expected to be good targets for protein functionalization. In our studies so far, we have found that (1) hemin oxidatively activates luminol derivatives as a catalyst, (2) N-Me luminol derivative specifically forms a covalent bond with a tyrosine residue among the 20 kinds of natural amino acid residues, and (3) the efficiency of tyrosine labeling with N-Me luminol derivative is markedly improved by using horseradish peroxidase (HRP) as a catalyst. We were able to use mol. oxygen as an oxidant under HRP/NADH conditions. By using these methods, the functionalization of purified proteins was carried out. Because N-Me luminol derivative is an excellent protein labeling reagent that responds to the activation of peroxidase, this new method is expected to open doors to such biol. applications as the signal amplification of HRP-conjugated antibodies and the detection of protein association in combination with peroxidase-tag technol. In the experiment, the researchers used many compounds, for example, 2-Methyl-2,3-dihydrophthalazine-1,4-dione (cas: 18393-54-9Recommanded Product: 18393-54-9).
2-Methyl-2,3-dihydrophthalazine-1,4-dione (cas: 18393-54-9) belongs to phthalazine derivatives. Although, N-containing heterocyclic compounds are widely distributed in nature, the presence of two adjacent nitrogen atoms in the phthalazine ring makes it rare and not very familiar in isolated extracts from living organisms. As with pyridazines, phthalazines, the other benzopyridazines, were also prepared most frequently through the condensation of hydrazines with carbonyl-containing compounds, typically phthalate derivatives. Recommanded Product: 18393-54-9
Referemce:
Phthalazine – Wikipedia,
Phthalazine | C8H6N2 – PubChem